Determination of 9 Plant Growth Regulator Residues in Whole Bananas, Pulp, and Leaves by QuEChERS Syringe Filter Purification and UPLC-MS/MS Method
14/07/20241.Institute for Agricultural Product Quality Safety and Testing Technology, Guangxi Academy of Agricultural Sciences, Quality Inspection and Test Center for Sugarcane and Its Product, China Ministry of Agriculture (Nanning), Nanning 530007, China2.Biotechnology Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, ChinaModern Food Science and Technology, 2022, Vol.38, No.11 Abstract: A method was developed for the simultaneous determination of 9 plant growth regulator residues ((PGRs), such as uniconazole and paclobutrazol, in whole banana fruit, banana pulp, and banana leaves by combining UPLC-MS/MS and QuEChERS syringe filter methodology, to provide a reference for the simultaneous determination of multiple PGR residues in…A Rapid Method to Detect 53 Pesticide Residues/Multi-Pesticide Residues in Strawberries Using LC-MS/MS and QuEChERS Syringe Filter
14/07/2024Zhenjiang Agricultural Products Quality Inspection and Testing Center, Zhenjiang, Jiangsu, China Abstract: A rapid detection method for 45 pesticides and 8 metabolites (53 Pesticide Residues，Multi-Pesticide Residues) including fluazinam and chlorotoluron in strawberries was established using a multifunctional syringe filter and LC-MS/MS based on QuEChERS preprocessing technology. Strawberry samples were extracted with 1% acetonitrile containing acetic acid, purified and filtered using a QuEChERS syringe filter, and separated on a Waters BEH C18 column. Detection was performed using MRM mode on mass spectrometry. Results showed excellent linearity (R² > 0.99) for different target compounds at three fortification levels (0.05, 0.10, 0.20 mg/kg), with…Fastest QuEChERS Kit from MoxLab
12/07/2024What Is QuEChERS Method QuEChERS (Quick, Easy, Cheap, Effective, Rugged, and Safe) is a sample preparation method that was initially developed in 2003 for analyzing veterinary drugs in food. The method was also tested for pesticide residue analysis in plant material with great success. In 2003, the detailed method was published in the journal AOAC International. Since then, the QuEChERS method has evolved into a sample preparation technique used on a variety of analytes in an expanding number of sample types before analysis by GC-MS or LC-MS. What Is The Advantage of QuEChERS Method (1) High recovery rates, with recovery exceeding 85% for a wide range of polar…QuEChERS Kit Application: Rapid Analysis of Carbamate Pesticide Residues in Cabbage by HPLC
12/07/2024Use QuEChERS Kit to simplify the purification process A rapid detection method for 10 kinds of carbamate pesticide in cabbage was established by extracting high performance liquid chromatography according AOAC standard, in which a kind of creative QuEChERS kit-QuSLE syringe filters were involved to simplify the purification process to one step. The sample was extracted with acetonitrile, and the extracts were concentrated by nitrogen blowing, cleaned up by QuEChERS syringe filter and analyzed. The recovery rate and precision were measured by standard recovery method, and the accuracy and reliability of the method were tested. The results showed that the recoveries…
Determination of 9 Plant Growth Regulator Residues in Whole Bananas, Pulp, and Leaves by QuEChERS Syringe Filter Purification and UPLC-MS/MS Method
14/07/20241.Institute for Agricultural Product Quality Safety and Testing Technology, Guangxi Academy of Agricultural Sciences, Quality Inspection and Test Center for Sugarcane and Its Product, China Ministry of Agriculture (Nanning), Nanning 530007, China2.Biotechnology Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, ChinaModern Food Science and Technology, 2022, Vol.38, No.11 Abstract: A method was developed for the simultaneous determination of 9 plant growth regulator residues ((PGRs), such as uniconazole and paclobutrazol, in whole banana fruit, banana pulp, and banana leaves by combining UPLC-MS/MS and QuEChERS syringe filter methodology, to provide a reference for the simultaneous determination of multiple PGR residues in…A Rapid Method to Detect 53 Pesticide Residues/Multi-Pesticide Residues in Strawberries Using LC-MS/MS and QuEChERS Syringe Filter
14/07/2024Zhenjiang Agricultural Products Quality Inspection and Testing Center, Zhenjiang, Jiangsu, China Abstract: A rapid detection method for 45 pesticides and 8 metabolites (53 Pesticide Residues，Multi-Pesticide Residues) including fluazinam and chlorotoluron in strawberries was established using a multifunctional syringe filter and LC-MS/MS based on QuEChERS preprocessing technology. Strawberry samples were extracted with 1% acetonitrile containing acetic acid, purified and filtered using a QuEChERS syringe filter, and separated on a Waters BEH C18 column. Detection was performed using MRM mode on mass spectrometry. Results showed excellent linearity (R² > 0.99) for different target compounds at three fortification levels (0.05, 0.10, 0.20 mg/kg), with…How to Sterilize A MicroPipette
21/07/2024Why We Need to Sterilize A MicroPipette Although pipettes are small, as a common laboratory instrument, they play a crucial role in the accuracy and precision of our experimental results. Therefore, to improve our experimental outcomes, this article will discuss the disinfection and sterilization of pipettes. For pipettes, the concepts of disinfection and sterilization are often confused. In fact, there is a clear difference between the two: disinfection merely requires controlling the viable bacteria on the pipette within a certain range to achieve a harmless level; sterilization, on the other hand, is more stringent and requires the elimination of all…How to Detect the Concentration of Nitrosamines in Electronic Cigarette Emissions
28/05/2024Syringe Filter to Filtrate Solution of Electronic Cigarette Emissions Cambridge filter paper: 44mm Ammonium acetate: Analytical grade Centrifuge tubes: 50ml Pure water: Resistivity of 18.2 MΩ Syringe filter: PTFE, 0.22μm Experimental Conditions to Analysis the Concentration of Nitrosamines in Electronic Cigarette Emissions LC-MS/MS Waters Xeov TQD HPLC column ACQUITY UPLC BEH C18 1.7μm Collection Mode MRM Ionization Mode Positive Ion Mode Substance NNN NAT NAB NNK Parent Ion/Cone Voltage (V) 178/25 190.1/13 192.1/15 208.1/15 Quantitative Ion/Collision Energy (V) 148/8 160/10 162/10 122/10 Qualitative Ion/Collision Energy (V) 120/25 106/15 133/25 79/30 Injection Volume 1.0 µL Ion Source Temperature 150°C Desolvation Gas Temperature…Fastest QuEChERS Kit from MoxLab
12/07/2024What Is QuEChERS Method QuEChERS (Quick, Easy, Cheap, Effective, Rugged, and Safe) is a sample preparation method that was initially developed in 2003 for analyzing veterinary drugs in food. The method was also tested for pesticide residue analysis in plant material with great success. In 2003, the detailed method was published in the journal AOAC International. Since then, the QuEChERS method has evolved into a sample preparation technique used on a variety of analytes in an expanding number of sample types before analysis by GC-MS or LC-MS. What Is The Advantage of QuEChERS Method (1) High recovery rates, with recovery exceeding 85% for a wide range of polar…How To Remove Bacteria in Biological Samples？——Escherichia Coli Solution Filtration
28/05/2024Why is a syringe filter used in cell culture? The syringe filter typically features micropores that effectively block the passage of bacteria, fungi, and other microorganisms, ensuring the sterility of the culturing process. This helps maintain the purity of cell culture and prevents potential contamination from affecting experimental results. In the process of cell culture, all operations require sterility. Common solutions such as PBS and ultrapure water can be sterilized by high-pressure sterilization, while there are some drugs or other reagents are temperature-sensitive. In this situation, syringe filter is a convenient and rapid method to remove bacteria. In this experiment,…Why We Need Syringe Filters In Pharmaceutical Field
17/07/2024Syringe filter is the Gateway to analysis systems which provide a protection! The microporous filter membrane is created by coating a support layer with high-molecular-weight chemical materials and pore-forming additives after special treatment. It is mainly used for filtering the mobile phase and samples in chromatographic analysis, with its function manifested in the following two aspects: 1.Pharmaceutical Syringe Filters Protect Chromatographic Columns and Liquid Phase Injection Systems Chromatographic Columns: The small particles in solvents and samples can clog the chromatographic column and frits due to the extremely small interstitial spaces within the column, as the packing material particles are very…QuEChERS Kit Application: Rapid Analysis of Carbamate Pesticide Residues in Cabbage by HPLC
12/07/2024Use QuEChERS Kit to simplify the purification process A rapid detection method for 10 kinds of carbamate pesticide in cabbage was established by extracting high performance liquid chromatography according AOAC standard, in which a kind of creative QuEChERS kit-QuSLE syringe filters were involved to simplify the purification process to one step. The sample was extracted with acetonitrile, and the extracts were concentrated by nitrogen blowing, cleaned up by QuEChERS syringe filter and analyzed. The recovery rate and precision were measured by standard recovery method, and the accuracy and reliability of the method were tested. The results showed that the recoveries…
How to Perform Quality Control on a Syringe Filter？Bubble Point Testing
04/06/2026The microporous membrane filtration method, as an important means of sterilization and particle removal, has been widely used in pharmaceuticals, particle detection, and sample processing. Syringe filter, as one of the most common laboratory consumables, in addition to undergo dissolution testing and chemical compatibility testing, integrity testing should also be performed when performing Quality Control on a Syringe FilterThe integrity of the laboratory syringe filter is a crucial indicator in filtration products. Whether a filtration system can maintain its integrity before and after filtration is an important parameter for assessing the quality of a filtration product. For filtration processes, especially sterilizing-grade filtration, integrity testing is a…How to Calibrate A Pipette? Simplest Way for Pipette Calibration
21/07/2024Here's a trick to calibrate your pipette How Often A Pipette Calibration Should be Conducted? The pipette calibration generally needs to be conducted once a year, and if it is used frequently, calibration needs to be more frequent as well Here's A Trick to Calibrate A Pipette Have you ever wondered whether the pipette you're using is accurate or not? How do we verify the accuracy of our pipettes? And what should we do if the pipette is not accurate?First, let's take a look at the errors of different pipettes, as shown in the table below: The Permissible Error and…An Experiment to Help You Understand Filtration Efficiency!
01/06/2024Filtration Efficiency Filtration Efficiency is usually stated in terms of the percentage of particles of a certain size that would be stopped and retained by a filter medium. What Is The Filtration Retention of A Laboratory Syringe Filter? The retention rate is an important parameter for the Laboratory Syringe Filter. When using a filter for filtration, undesired components are often captured by the filter. The retention rate represents the ability of the membrane to either prevent certain components from passing through or retain a specific component in the liquid. Due to different calculation formulas, various numerical values can be obtained.The…How to Use A Syringe Filter – Most Easy Guide-4 Steps to Use A Syringe Filter
21/07/2024How to Use A Syringe Filter? Step 1 Before filling with sample, draw approximately 1 mL of air into the syringe. This will minimize fluid retention. Step 2 Draw your sample into the syringe, then draw in about mL of air. Invert the syringe and wipe residue off tip. Step 3 Connect the syringe to the syringe filter using a interconnection. Twist gently tonsure a secure seal. Step 4 Filter syringe contents into a vial.Afterwards, remove the syringe filter, draw air into the syringe,re-attach the syringe filter, and press the plunger to filter the residual sample. This maximize sample recovery.0.22μm Syringe Filter VS. 0.45 μm Syringe Filter: How To Make A Choice
21/07/20240.22μm Syringe Filter: With Sterilizing-grade Membrane Inside With a higher filtration precision, it can filter out microorganisms, cells, etc., with a diameter larger than 0.22 micrometers. It is generally used to remove extremely fine particles in samples or mobile phases, meeting the requirements of sterilization at 99.99% according to GMP or pharmacopeia standards. Therefore, it is called a sterile filtration membrane and is commonly used in the pharmaceutical, medical, and food industries. Compared to membranes with larger pore sizes, the 0.22μm membrane has higher resistance during filtration, requiring higher pressure to allow the liquid to pass through the membrane pores.…Hydrophobic PTFE Syringe Filter Vs Hydrophilic PTFE Syringe Filter: What Is the Difference
21/07/2024What Is PTFE Syringe Filter/Teflon Syringe Filter Polytetrafluoroethylene, abbreviated as PTFE is a polymer synthesized by polymerizing tetrafluoroethylene monomers. It is white, waxy, semi-transparent, and exhibits excellent heat and cold resistance, allowing for long-term usage in the temperature range of -180 to 260ºC.The PTFE syringe filter, also known as a Teflon filter, utilizes an internal PTFE micro-porous membrane. This material consists of micro-pores created by billions of interconnected fibers arranged in a non-concentric cross pattern. The PTFE syringe filter exhibits features including air permeability without water permeability, high air permeability, low resistance, a high particle retention rate, excellent temperature resistance,…What Is The Dead Volume of A Syringe Filter
28/05/2024The dead volume reflects the extent of volume loss in a sample. During sample filtration, some residual sample is inevitably left inside the filter or filter membrane, affecting the final sample recovery rate. The smaller the dead volume, the less the loss, and the higher the recovery rate. The dead volume refers to the portion of volume that cannot be recoveredHow Does The Laboratory Syringe Filter Affects The Filtration Results?
01/06/2024How Does The Laboratory Syringe Filter Affects the Filtration Results? Here, a Chinese engineer conducted relevant experiments. This engineer selected filters with five different membrane materials, designed a series of experiments, and observed chromatograms of the same solution passing through these five filters. Additionally, they prepared different solutions to observe the chromatographic performance of each solution after passing through different filters. So, what are the experimental results? Let's take a look! The Laboratory Syringe Filters Used in His Experiment Filter Membrane Material Aperture /μm Diameter 1 Nylon 66 0.22 13 2 Nylon 0.45 13 3 Polyether Sulfone (PES) 0.22 13…Can A Laboratory Syringe Filter Be Reused?
01/06/2024Laboratory syringe filters are designed for disposable use . Reuse poses a high risk of cross-contamination. There is literature indicating that the repeated use of Syringe filters would lead to a decrease in measurement precision.How to Choose A Syringe Filter？
18/06/20243 Most Importants Points for Syringe Filter Selection How to Choose A Syringe Filter？ Consider Membrane Pore Size 0.45μm Used for general sample mobile phase filtration, suitable for typical chromatography requirements. For example, used in chromatography systems with 3μm or larger particle size fillers. 0.22μm Meets the requirement to remove extremely fine particles from samples and mobile phases. For example, used in chromatography systems with fillers less than 3μm. 1-5μm Commonly used for challenging turbid solutions. Pre-filtering with a 1-5μm membrane is recommended before using the corresponding membrane for final filtration. How to Choose A Syringe Filter？Consider Diameter of…