How to Detect the Concentration of Nitrosamines in Electronic Cigarette Emissions

Syringe Filter to  Filtrate Solution of Electronic Cigarette Emissions 

Cambridge filter paper: 44mm

Ammonium acetate: Analytical grade

Centrifuge tubes: 50ml

Pure water: Resistivity of 18.2 MΩ

Syringe filter: PTFE, 0.22μm

Experimental Conditions to Analysis the Concentration of Nitrosamines in Electronic Cigarette Emissions

 

LC-MS/MSWaters Xeov TQD
HPLC columnACQUITY UPLC BEH C18 1.7μm
Collection ModeMRM
Ionization ModePositive Ion Mode
SubstanceNNNNATNABNNK
Parent Ion/Cone Voltage (V)178/25190.1/13192.1/15208.1/15
Quantitative Ion/Collision Energy (V)148/8160/10162/10122/10
Qualitative Ion/Collision Energy (V)120/25106/15133/2579/30
Injection Volume1.0 µL
Ion Source Temperature150°C
Desolvation Gas Temperature550°C
Desolvation Gas Flow Rate1000L/Hr
Carrier GasNitrogen Gas
Flow Rate0.3 ml/min
Mobile PhaseC:2mmol/LAmmonium Acetate  B:Methanol with 0.01% Formic Acid
Elution Program0 min(1%B+99%C)→3.0min(90%B+10%C)→4.0min(90%B+10%C)→4.1min(1%B+99%C)→7.0min(1%B+99%C)

Analysis Procedure:

  • Wipe the fixture with isopropanol and place it into the Cambridge filter paper.
  • Connect the electronic cigarette and perform suction under the specified conditions.
  • After completing 100 suctions, carefully remove the Cambridge filter paper and place it in a centrifuge tube.
  • Wipe the fixture with two quarters of Cambridge filter paper, combine them, and insert them into the aforementioned centrifuge tube.
  • Add 20 ml of ammonium acetate solution (100 mM) to the centrifuge tube, and mechanically oscillate at a speed of 210 rpm for 60 minutes.
  • Filter the mixture using a PTFE syringe filter, and analyze the filtrate using LC-MS/MS.

Leave a Comment

Your email address will not be published. Required fields are marked *

Shopping Cart
MoxLab