3 Most Importants Points for Syringe Filter Selection
How to Choose A Syringe Filter？ Consider Membrane Pore Size
|
0.45μm |
Used for general sample mobile phase filtration, suitable for typical chromatography requirements. For example, used in chromatography systems with 3μm or larger particle size fillers. |
|
0.22μm |
Meets the requirement to remove extremely fine particles from samples and mobile phases. For example, used in chromatography systems with fillers less than 3μm. |
|
1-5μm |
Commonly used for challenging turbid solutions. Pre-filtering with a 1-5μm membrane is recommended before using the corresponding membrane for final filtration. |
How to Choose A Syringe Filter？Consider Diameter of Syringe Filter
|
ID |
Sample Volume |
|
Φ13mm |
2-10mL |
|
Φ25 mm |
10-100mL |
How to Choose A Syringe Filter？Consider Membrane Material
|
Solvent Properties |
Membrane Material |
Applicability |
|
Aqueous System |
Cellulose acetate(CA) CA Syringe Filter |
Low protein adsorption, good hydrophilicity, strong chemical compatibility; pH range of 3-7; suitable for filtering antibiotics, globulins, vaccine sera, and tissue culture media: liquid sterilization, particulate filtration, and other water-based samples |
|
Aqueous System |
Polyethersulfone (PES) |
Hydrophilic membrane with high flux, exhibiting excellent throughput: Low protein adsorption, enhancing protein recovery; Aging-resistant, good resistance to deformation, excellent chemical resistance, suitable for high-temperature liquid filtration. It is applicable in the pharmaceutical industry for the filtration of biologics and sera, terminal filtration of large intravenous antibiotics, and in the food industry for terminal filtration of beverages, wines, as well as ultra-pure water terminal filtration, among others. |
|
Aqueous System |
Polyvinylidene Fluoride (PVDF) 0.22μm Hydrophilic PVDF Syringe Filter 0.45 μm |
Hydrophilic, aging-resistant, and solvent-resistant with excellent performance; can be used for long periods between -40°C and 150°C, with low protein adsorption. Resistant to corrosion from various strong acids, bases, and solvents at room temperature. Suitable for filtering general biological samples twice, as well as samples requiring high protein recovery. Soluble in strong polar solvents such as DMAC and NMP. |
|
Aqueous System |
Mixed Cellulose Ester Membrane (MCE) |
High porosity, excellent retention performance; good hydrophilicity, high water flux; not resistant to organic solvents and strong acid/base solutions. Possesses characteristics of good mechanical strength, strong thermal stability, and low adsorption. Suitable for filtration applications in laboratories and small-scale production processes, such as sterilization and removal of particles in culture media and culture liquids. |
|
Organic system |
Hydrophobic Polytetrafluoroethylene (PTFE) |
Filterable for almost all organic solvents, with high mechanical strength, resistant to high-temperature liquids, suitable for filtering air and particulate impurities. Unless pre-wetted, filtering water solutions may be difficult to advance through the filter sample due to tension hindrance. |
|
Aqueous /organic system |
Nylon |
Used for filtering alkaline solutions and organic solvents: Nylon membranes have excellent mechanical strength and strong adsorption, can withstand most organic solvents and many alkaline solutions, especially suitable for filtering alkaline solutions. For filtering organic solvents, such as particle removal in HPLC mobile phases, nylon membranes are more cost-effective than PTFE membranes. Due to the relatively high adsorption performance of nylon membranes, they are generally not recommended for filtering culture media or biological samples such as protein solutions to avoid sample loss due to adsorption. In such cases, a low-adsorption cellulose acetate membrane (CA) is usually more suitable. It is the most economical choice in the laboratory, not only for filtering water-based samples but also compatible with most organic solvents, excluding filtration of acid solutions and solutions containing halogenated hydrocarbons and aromatic hydrocarbons. |
|
Aqueous /organic system |
Hydrophilic Polytetrafluoroethylene (PTFE) |
Universal membrane with high mechanical strength, capable of withstanding high-temperature liquids, and resistant to filtering air and particulate impurities. Compatible with all acid and alkali solutions, making it the optimal choice for demanding laboratory applications. |
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