Why We Need to Sterilize A MicroPipette
How to Sterilize A MicroPipette
1. For Pipettes that Cannot Be Fully Sterilized
First, wrap the parts that need to be sterilized in sterilization bags, aluminum foil, or kraft paper. Sterilize them at 121°C (1 bar of pressure) for 20 minutes. After sterilization, let them air dry at room temperature, lubricate the piston, and then reassemble the pipette.
2. For Pipettes that Can Be Fully Sterilized
The entire pipette can be placed at 121°C (1 bar of pressure) for 20 minutes for full high-temperature, high-pressure sterilization.
3. For Pipettes that Cannot Be Sterilized at High Temperature and Pressure
If the pipette cannot be sterilized at high temperatures and pressure but needs to be used for special operations, such as RNA extraction, and you are concerned about contamination affecting the experimental results, clean the pipette tip with 75% ethanol. If the pipette is used for RNA extraction, use 75% ethanol that is RNase-free.
Note: Pipettes that can only be partially sterilized should not be fully sterilized, as this can cause discoloration of the reading section and inaccurate measurements.
