Why is a syringe filter used in cell culture?
The syringe filter typically features micropores that effectively block the passage of bacteria, fungi, and other microorganisms, ensuring the sterility of the culturing process. This helps maintain the purity of cell culture and prevents potential contamination from affecting experimental results.
In the process of cell culture, all operations require sterility. Common solutions such as PBS and ultrapure water can be sterilized by high-pressure sterilization, while there are some drugs or other reagents are temperature-sensitive. In this situation, syringe filter is a convenient and rapid method to remove bacteria. In this experiment, Escherichia coli bacterial solution is simulated to be a solution that needs to be processed
How does a syringe filter remove bacteria?
1. Experimental Objective
Bacteria Removal
2. Product Selection
MoxLab 0.22μm PVDF Syringe filter （sterile）.
The average diameter of bacteria is 0.5~5μm, and a 0.22μm filter can effectively filter and remove them. Other Instrument Consumables NIKON ECLIPSE TS2 microscope, 1mL of disposable sterile syringe, 1.5ml EP tube) (BIOFIL), 96 well plate (BIOFIL), 1mL of pipette (Eppendorf), DH5α bacterial culture (after overnight shaking).
3.Experimental Procedure
Draw approximately 2mL of Escherichia coli bacterial solution with a syringe. Connect the syringe to a 0.22μm PVDF filter and press the plunger to pass the Escherichia coli bacterial solution through the 0.22μm filter. Collect the filtrate. Place the bacterial solution before and after filtration in a 96-well plate for microscopic observation to compare the quantity of Escherichia coli in the solution before and after filtration.
4. Result
 |
|
| Before filtration | After filtration |
| Much Escherichia coli was observed under the microscope, and the solution was turbid. | Little Escherichia coli, and the solution is clear and transparent. |
